摘要: Group living animals form striking aggregation patterns and display synchronization, polarization, and collective intelligence. Though many collective behavioral studies have been conducted on small animals like insects and fish, research on large animals is still rare due to the limited availability of field collective data. We used drones to record videos and analyzed the decision-making and behavioral spatial patterns in orientation of Kiang (Tibetan wild ass, Equus kiang). Leadership is unevenly distributed among Kiang, with the minority initiating majority behavior-shift decisions. Decisions of individual to join are driven by imitation between group members, and are largely dependent on the number of members who have already joined. Kiang respond to the behavior and position of neighbors through different strategies. They strongly polarize when moving, therefore adopting a linear alignment. When vigilant, orientation deviation increases as they form a tighter group. They remain scattered while feeding and, in that context, adopt a side-by-side alignment. This study reveals partially-shared decision-making among Kiang, whereby copying neighbors provides the wisdom to thrive in harsh conditions. This study also suggests that animals' spatial patterns in orientation depend largely on their behavioral states in achieving synchronization.

摘要: Argentine ants Linepithema humile are one of the most damaging invasive alien species worldwide. Enhancing or disrupting cognitive abilities, such as learning, has the potential to improve management efforts, for example by increasing preference for a bait, or improving ants' ability to learn its characteristics or location. Nectar-feeding insects are often the victims of psychoactive manipulation, with plants lacing their nectar with secondary metabolites such as alkaloids and non-protein amino acids which often alter learning, foraging, or recruitment. However, the effect of neuroactive chemicals has seldomly been explored in ants. Here, we test the effects of seven potential neuroactive chemicals-two alkaloids: caffeine and nicotine; two biogenic amines: dopamine and octopamine, and three nonprotein amino acids: beta-alanine, GABA and taurine-on the cognitive abilities of invasive L. humile using bifurcation mazes. Our results confirm that these ants are strong associative learners, requiring as little as one experience to develop an association. However, we show no short-term effect of any of the chemicals tested on spatial learning, and in addition no effect of caffeine on short-term olfactory learning. This lack of effect is surprising, given the extensive reports of the tested chemicals affecting learning and foraging in bees. This mismatch could be due to the heavy bias towards bees in the literature, a positive result publication bias, or differences in methodology.

摘要: Visual cues are important in both interspecific and intraspecific communication. The species confidence hypothesis proposes that animals are more attracted to conspecific colors and repelled by colors, not on their bodies. Studies on terrestrial lizards and birds have tested the species confidence hypothesis and shown that conspecific colors elicit reduced antipredator behavior. To date, the species confidence hypothesis has not been tested in the marine environment, specifically on coral reefs where color communication is of vital importance. We addressed this knowledge gap by measuring flight initiation distance (the distance an individual moves away from an approaching threat) in dusky damselfish (Stegastes nigricans) in response to an approaching disc of 1 of 4 different color treatments: conspecific, blue, yellow, and black. If the species confidence hypothesis explained variation in damselfish flight initiation distance, then we expected individuals to tolerate closer approaches when approached by a conspecific color. In addition, we calculated the color difference between each stimulus and its corresponding background as a potential alternative explanation for flight responses. Damselfish tolerated the closest approach from the conspecific color stimulus; there were no significant differences between other colors and there was no support for the alternative color difference hypothesis. As with similar terrestrial studies, these results are relevant to ecotourists' choice of swimsuit and wetsuit colors because color choice may modify natural antipredator behavior.

摘要: Movement is an important animal behavior contributing to reproduction and survival. Animal movement is often examined in arenas or enclosures under laboratory conditions. We used the red flour beetle (Tribolium castaneum) to examine here the effect of the arena size, shape, number of barriers, access to the arena's center, and illumination on six movement properties. We demonstrate great differences among arenas. For example, the beetles moved over longer distances in clear arenas than in obstructed ones. Movement along the arena's perimeter was greater in smaller arenas than in larger ones. Movement was more directional in round arenas than in rectangular ones. In general, the beetles stopped moving closer to the perimeter and closer to corners (in the square and rectangular arenas) than expected by chance. In some cases, the arena properties interacted with the beetle sex to affect several movement properties. All these suggest that arena properties might also interact with experimental manipulations to affect the outcome of studies and lead to results specific to the arena used. In other words, instead of examining animal movement, we in fact examine the animal interaction with the arena structure. Caution is therefore advised in interpreting the results of studies on movement in arenas under laboratory conditions and we recommend paying attention also to barriers or obstacles in field experiments. For instance, movement along the arena's perimeter is often interpreted as centrophobism or thigmotaxis but the results here show that such movement is arena dependent.

摘要: Human disturbances are considered to break reproduction barriers among species. Significant increases in hybridization events have been reported among a large number of taxonomic groups in anthropogenic environments, providing novel insights into species evolution mechanisms and conservation management in the Anthropocene. The Eastern Golden Frog (Pelophylax plancyi) and BlackSpotted Frog (P. nigromaculatus) are two sympatric anuran species with a long history of mitochondrial genome introgression in highly urbanized continental East Asia. However, there is only limited understanding of the pattern of their contemporary hybridization and factors influencing their interspecific relationship under anthropogenic disturbances. Here, interspecific hybridization between P. plancyi and P. nigromaculatus at the population level was investigated in Shanghai. All except two haplotypes obtained from both species in Shanghai were mixed together, and located in the introgression clade, implying multiple ancient mitochondrial introgression events occurred in the populations of our study area. Asymmetric genetic introgression was detected by microsatellite markers, with 0.7% of P. plancyi and 14.6% of P. nigromaculatus identified as contemporary admixed individuals. Consistent with the trend of population density, higher genetic diversity of neutral microsatellite loci was found in the more abundant P. plancyi; however, variation in mitochondrial (Cyt-b) and nuclear (POMC) genes was higher in relatively rare P. nigromaculatus. The population density of P. plancyi and number of water patches within local habitats were significantly positively correlated with both occurrences and proportions of admixed individuals in the populations of P. plancyi and P. nigromaculatus. Considering the prevalent transformation of habitats in urbanized areas, these results imply that a high population density in isolated artificially altered habitats is likely to increase interspecific hybridization. Thus, population monitoring and improvement of landscape connectivity between habitats would be needed to control the intensity of interspecific hybridization between P. plancyi and P. nigromaculatus in anthropogenic-disturbed environments.