摘要: In sexually dimorphic species characterized by exaggerated male ornamentation, behavioral isolation is often attributed to female preferences for conspecific male signals. Yet, in a number of sexually dimorphic species, male mate choice also results in behavioral isolation. In many of these cases, the female traits that mediate species boundaries are unclear. Females in sexually dimorphic species typically lack many of the elaborate traits that are present in males and that are often used for taxonomic classification of species. In a diverse and largely sexually dimorphic group of fishes called darters (Percidae: Etheostoma), male mate choice contributes to behavioral isolation between a number of species; however, studies addressing which female traits males prefer are lacking. In this study, we identified the dominant female pattern for two sympatric species, Etheostoma zonate and Etheostoma barrenense, using pattern energy analysis, and we used discriminate function analysis to identify which aspects of female patterning can reliably classify species. We then tested the role of female features in male mate choice for E. zonate, by measuring male preference for computer animations displaying the identified (species-specific) conspecific features. We found that the region above the lateral line is important in mediating male mate preferences, with males spending a significantly greater proportion of time with animations exhibiting conspecific female patterning in this region than with animations exhibiting heterospecific female patterning. Our results suggest that the aspects of female phenotypes that are the target of male mate choice are different from the conspicuous male phenotypes that traditionally characterize species.

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摘要: Foraging, as an energy-consuming behavior, is very important for colony survival in termites. How energy metabolism related to glucose decomposition and adenosine triphosphate (ATP) production influences foraging behavior in termites is still unclear. Here, we analyzed the change in energy metabolism in the whole organism and brain after silencing the key metabolic gene isocitrate dehydrogenase (IDH) and then investigated its impact on foraging behavior in the subterranean termite Odontotermes formosanus in different social contexts. The IDH gene exhibited higher expression in the abdomen and head of O. formosanus. The knockdown of IDH resulted in metabolic disorders in the whole organism. The dsIDH-injected workers showed significantly reduced walking activity but increased foraging success. Interestingly, IDH knockdown altered brain energy metabolism, resulting in a decline in ATP levels and an increase in IDH activity. Additionally, the social context affected brain energy metabolism and, thus, altered foraging behavior in O. formosanus. We found that the presence of predator ants increased the negative influence on the foraging behavior of dsIDH-injected workers, including a decrease in foraging success. However, an increase in the number of nestmate soldiers could provide social buffering to relieve the adverse effect of predator ants on worker foraging behavior. Our orthogonal experiments further verified that the role of the IDH gene as an inherent factor was dominant in manipulating termite foraging behavior compared with external social contexts, suggesting that energy metabolism, especially brain energy metabolism, plays a crucial role in regulating termite foraging behavior.

摘要: Sexual development in insects is regulated by a complicated hierarchical cascade of sex determination. The primary signals are diverse, whereas the central nexus doublesex (dsx) gene is relatively conserved within the pathway. Aedes (Stegomyia) albopictus is an important vector with an extensive worldwide distribution. We previously reported that Ae. albopictus dsx (Aalbdsx) yields one male- (Aalbdsx(M)) and three female-specific isoforms (Aalbdsx(F1-3)); however, the spatiotemporal expression profiles and mechanisms regulating sex-specific alternative splicing require further investigation. In this study, we demonstrated that the Aalbdsx(M) messenger RNA (mRNA) represents the default pattern when analyzed in human foreskin fibroblasts and HeLa cells. We combined reverse transcription polymerase chain reaction with RNA immunoprecipitation using specific antibodies against tagged Ae. albopictus male-determining factor AalNix and confirmed that AalNix indirectly regulates dsx pre-mRNA and regulates its alternative splicing. During the early embryo stage (0-2 and 4-8 h), maternal dsx(F) and default splicing dsx(M) were detected in both sexes; the expression of dsx(M) then decreased until sufficient AalNix transcripts accumulated in male embryos at 20-24 h. These findings suggest that one or more potential dsx splicing enhancers can shift dsx(M) to dsx(F) in both sexes; however, the presence of Nix influences the function of this unknown splicing enhancer and ultimately leads to the formation of dsx(M) in males. Finally, our results provide important insight into the regulatory mechanism of dsx alternative splicing in the mosquito.

摘要: Parental care directed to adult offspring is uncommon in animals. Such parental care has been documented in Xylocopinae bees (Hymenoptera: Apidae). Moreover, someCeratinabees (Xylocopinae) are known to feed mature siblings, and feeding of mature siblings is achieved by dwarf eldest daughters when mothers died. These daughters are intentionally malnourished by mothers and usually originate from the first brood cell. Here, we examined the pattern of care provided to young adults in three small European carpenter bees:Ceratina(Ceratina)cucurbitina,C. (Euceratina)chalybea, andC. (E.)nigrolabiata. Observations of nest departures and arrivals were performed to study foraging behavior. We detected intensive foraging behavior of mothers in all three studied species. However, we did not observe regular foraging behavior of daughters in any species. The experimental removal of mothers inC. cucurbitinaled to the emigration of young adults and did not initiate foraging activity in daughters. We conclude that the feeding of siblings does not occur in these species unlike in the American speciesC. calcarata. We detected female-biased sex ratios in the first brood cell inC. cucurbitinaandC. chalybea. Female offspring in the first brood cell was smaller than other female offspring only inC. cucurbitina. Our results show that a female-biased sex ratio and the small size of daughters in the first brood cell do not provide sufficient evidence for demonstrating the existence of an altruistic daughter and also that the pattern of maternal investment is not exclusively shaped by social interactions.