摘要: Duetting, or the stereotypical, repeated and often coordinated vocalizations between 2 individuals arose independently multiple times in the Order Primates. Across primate species, there exists substantial variation in terms of timing, degree of overlap, and sex-specificity of duet contributions. There is increasing evidence that primates can modify the timing of their duet contributions relative to their partner, and this vocal flexibility may have been an important precursor to the evolution of human language. Here, we present the results of a fine-scale analysis of Gursky's spectral tarsier Tarsius spectrumgurskyae duet phrases recorded in North Sulawesi, Indonesia. Specifically, we aimed to investigate individual-level variation in the female and male contributions to the duet, quantify individual- and pair-level differences in duet timing, and measure temporal precision of duetting individuals relative to their partner. We were able to classify female duet phrases to the correct individual with an 80% accuracy using support vector machines, whereas our classification accuracy for males was lower at 64%. Females were more variable than males in terms of timing between notes. All tarsier phrases exhibited some degree of overlap between callers, and tarsiers exhibited high temporal precision in their note output relative to their partners. We provide evidence that duetting tarsier individuals can modify their note output relative to their duetting partner, and these results support the idea that flexibility in vocal exchanges-a precursor to human language-evolved early in the primate lineage and long before the emergence of modern humans.

摘要: Immunofluorescence has been widely used to localize microbes or specific molecules in insect tissues or cells. However, significant autofluorescence is frequently observed in tissues which can interfere with the fluorescent identification of target antigens, leading to inaccurate or even false positive fluorescent labeling. The alimentary canal of the potato psyllid, Bactericera cockerelli Sulc, exhibits intense autofluorescence, hindering the application of immunolocalization for the detection and localization of the economically important pathogen transmitted by this insect, Candidatus Liberibacter solanacearum (Lso). In the present study, we tested the use of irradiation, hydrogen peroxide (H2O2) and Sudan black B (SBB) treatments to reduce the autofluorescence in the B. cockerelli alimentary canal tissues. Furthermore, we assessed the compatibility of the above-mentioned treatments with Lso immunolocalization and actin staining using phalloidin. Our results showed that the autofluorescence in the alimentary canal was reduced by irradiation, H2O2, or SBB treatments. The compatibility assays indicated that irradiation and H2O2 treatment both greatly reduced the fluorescent signal associated with Lso and actin. However, the SBB incubation preserved those target signals, while efficiently eliminating autofluorescence in the psyllid alimentary canal. Therefore, herein we propose a robust method for reducing the autofluorescence in the B. cockerelli alimentary canal with SBB treatment, which may improve the use of immunofluorescence labeling in this organism. This method may also have a wide range of uses by reducing the autofluorescence in other arthropod species.

摘要: The genome-wide characterization of long non-coding RNA (lncRNA) in insects demonstrates their importance in fundamental biological processes. Essentially, an in-depth understanding of the functional repertoire of lncRNA in insects is pivotal to insect resources utilization and sustainable pest control. Using a custom bioinformatics pipeline, we identified 1861 lncRNAs encoded by 1852 loci in theSogatella furciferagenome. We profiled lncRNA expression in different developmental stages and observed that the expression of lncRNAs is more highly temporally restricted compared to protein-coding genes. More up-regulatedSogatella furciferalncRNA expressed in the embryo, 4th and 5th instars, suggesting that increased lncRNA levels may play a role in these developmental stages. We compared the relationship between the expression ofSogatella furciferalncRNA and its nearest protein gene and found that lncRNAs were more correlated to their downstream coding neighbors on the opposite strand. Our genome-wide profiling of lncRNAs inSogatella furciferaidentifies exciting candidates for characterization of lncRNAs, and also provides information on lncRNA regulation during insect development.

摘要: Sitodiplosis mosellana, a periodic but devastating wheat pest, relies on wheat spike volatiles as a cue in selecting hosts for oviposition. Insect odorant-binding proteins (OBPs) are thought to play essential roles in filtering, binding and transporting hydrophobic odorant molecules to specific receptors. To date, the molecular mechanisms underlying S. mosellana olfaction are poorly understood. Here, three S. mosellana antenna-specific OBP genes, SmosOBP11, 16 and 21, were cloned and bacterially expressed. Binding properties of the recombinant proteins to 28 volatiles emitted from wheat spikes were investigated using fluorescence competitive binding assays. Sequence analysis suggested that these SmosOBPs belong to the Classic OBP subfamily. Ligand-binding analysis showed that all three SmosOBPs preferentially bound alcohol, ester and ketone compounds, and SmosOBP11 and 16 also selectively bound terpenoid compounds. In particular, the three SmosOBPs had high binding affinities (K-i < 20 mu mol/L) to 3-hexanol and cis-3-hexenylacetate that elicited strong electroantennogram (EAG) response from female antennae. In addition, SmosOBP11 displayed significantly higher binding (K-i < 8 mu mol/L) than SmosOBP16 and 21 to 1-octen-3-ol, D-panthenol, alpha-pinene and heptyl acetate which elicited significant EAG response, suggesting that SmosOBP11 plays a major role in recognition and transportation of these volatiles. These findings have provided important insight into the molecular mechanism by which S. mosellana specifically recognizes plant volatiles for host selection, and have facilitated identification of effective volatile attractants that are potentially useful for pest monitoring and trapping.

摘要: MicroRNAs (miRNAs) are a class of short, non-coding transcripts that bind to 3 '-untranslated regions to trigger messenger RNA degradation or translational inhibition. Here we explored how miRNAs regulate sex determination in Bombyx mori, a lepidopteran model insect. Genes known to be involved in sex determination, BmPSI, Bmdsx, and BmMasc, are predicted targets of the species-specific miR-2738. Using a dual luciferase reporter assay in HEK293T cells, we confirmed that miR-2738 suppressed transcription of BmPSI, Bmdsx, and BmMasc. The levels of BmPSI and BmMasc were significantly down-regulated in B. mori miR-2738 overexpression. In contrast, the genetic disruption of miR-2738 using the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 transgenic system increased the levels of BmPSI and BmMasc transcripts, whereas splicing of Bmdsx was unaltered by miR-2738 depletion or overexpression. Taken together, this study implicates miR-2738 as a minor regulator of sex determination genes in the silkworm.