摘要: 根据高山姬鼠幼仔56d的生长资料初步分析了其生长发育规律;用电子天平测量了体重的增长过程;用开放式呼吸仪测定了静止代谢率(Resting metabolic rate,RMR)、非颤抖性产热(Nonshivering thermogenesis,NST)以及肺皮蒸发失水(Evaporative waterloss,EWL)。依据逻辑斯蒂曲线的拐点,高山姬鼠的体重生长可划分为加速生长相和减速生长相。幼仔的体温在17日龄前逐渐升高,35日龄时接近成体水平;静止代谢率和非颤抖性产热在17日龄前随日龄逐渐增大,17日龄后与体重成异速增长关系,RMR在49日龄时接近成体水平,NST在6日龄内即被激活;蒸发失水在断乳前较断乳后为高。高山姬鼠为典型的晚成性发育动物。高山姬鼠较短的妊娠期、较大的胎仔数、较长的哺乳期与其食物资源较丰富有关。

摘要: 克隆高原鼠兔脑红蛋白(Neuroglobin,NGB)基因编码区并检测其在成年高原鼠兔脑组织和其他组织中的表达,同时探讨高原鼠兔低氧适应的分子生物学机制。从高原鼠兔脑组织中提取总RNA,通过RT-PCR获得高原鼠兔NGB cDNA,将其与pGEM-TEasy载体连接,构建重组质粒,蓝白斑筛选阳性克隆并进行鉴定和测序;制备地高辛标记的RNA探针并采用原位杂交法(In suit hybridization,ISH)分析脑红蛋白基因在高原鼠兔脑组织中的分布;采用RT-PCR和蛋白印记(Westernblot)检测高原鼠兔不同组织中脑红蛋白的表达含量。将含有目的片段的阳性克隆经测序和Blast分析,显示其部分编码序列与GenBank中绵羊、大鼠等同源性很高(大于84%),表明本实验所克隆的序列为脑红蛋白基因;原位杂交结果显示NGB在青藏高原土著动物高原鼠兔脑部分布较为广泛;高原鼠兔不同组织中都有NGB mRNA表达,NGB基因并不是中枢神经系统所特有的,睾丸和肾上腺也有较高的表达。NGB基因在高原鼠兔脑组织和其他组织中分布较为广泛,推测NGB mRNA可能在高原鼠兔机体较为广泛的区域中发挥着作用,同时为高原低氧适应相关基因的研究提供了实验依据。

摘要: 小型哺乳动物能够通过行为策略的进化适应周围的环境。其中,种内社会组织也可随环境参数的变化而变化。河南灵宝程村气候干燥、海拔高,而新郑则气候湿润、海拔低。来自河南灵宝程村的棕色田鼠种群和来自新郑的种群是否在社会行为、情绪、以及性二型上有所差别,目前还不清楚。本研究中,我们通过旷场实验和熟悉选择试验比较了两个种群的情绪、运动能力以及对熟悉鼠和陌生鼠的选择。统计结果表明,程村种群的雌性个体比雄性个体重,而新郑种群的雄性体重比程村种群的重。在旷场实验中,程村种群比新郑种群表现出较多的焦虑样行为。配偶选择实验中,新郑种群不论雌雄都表现出了对陌生异性的喜好。程村种群雄性则更愿意选择熟悉的个体。而且,程村的雌性待在陌生箱攻击陌生鼠的时间远大于熟悉鼠。以上结果表明,两个野生种群在体重、情绪以及对熟悉鼠和陌生鼠选择上都表现出了明显的种群间差异。

摘要: From September to November 2007,we recorded and analyzed the echolocation calls of Ricketti's big-footed bat(Myotis ricketti) ,in call-recording cages using BatSound software.We also calculated measurements of wing shape for these bats. We compared the parameters of echolocation calls and wing shape between males and females.The results showed that only the interpulse interval was significantly different between males(68.49±10.99 ms) and females(83.61±13.77 ms) (t-test:t=-2.72,P<0.01) ,while the pulse duration(male:4.28±0.34 ms,female:4.64±0.97 ms) ,the dominant frequency(40.31±1.36 kHz,40.20±1.32 kHz) ,the max frequency(72.40±2.37 kHz,72.20±2.66 kHz) ,the min frequency(29.00±1.16 kHz,28.60±1.58 kHz) ,and the measurements of wing shape were not significantly different between males and females. M.ricketti had average wing loading(male:8.61±0.72 N/m2,female:8.51±0.81 N/m2) ,high aspect ratio(7.96±0.31,8.09±0.34) and high wing tip shape index(2.93±1.09,2.48±1.02) .

摘要: The plasmid cDNA library from the liver of Microtus fortis was constructed by using SMART technology. The titers of this cDNA library were 1076 pfu /μL,the recombination rate was about 94%,the library capacity was 1.08×106. 22 cDNAs chosen at random were sequenced. After BLAST analysis of these sequences,it was found that these cDNAs showed more than 75% similarity to the functional known genes of mice,rats and humans in the Genbank. The cDNA library was very important to the research of fatty liver-related gene screening and clones. These provided a base for further study on the molecular biology,fatty liver model development and pathogenesis research of Microtus fortis.