摘要: In order to investigate the effect of zedoary glucose injection on reproductive behavior in female mandarin voles(Microtus mandarinus),behavior focal sampling and immunohistochemistry methods were used to examine the relationships between behavior changes of female mandarin voles and estrogen receptor α immunoreactive neurons (ERα-IRs) in the ovary and in relevant brain regions after being fed with rabbit chow that was mixed with concentrations of 30 mg/kg zedoary glucose injection. Healthy adult female voles were divided into experimental and control groups. The experimental group was fed with the mixed chow and carrot,and control group was fed with rabbit chow and carrot. Behaviors of the adult female voles were observed after being fed for 30 days. The numbers of litters of these adult female voles after mating with adult male voles was recorded,and meanwhile the number of ERα-IRs in the relevant brain regions was detected. The results showed that:during the period of intraspecies female-male interactions in a cage,the female voles in the experimental group displayed more escape behaviors,defensive behaviors,and hostile attacks than did those in the control group. In addition,the number of litters born to the female voles in the experimental group was significantly less than to the control group,and the incubation period of females in the experimental group was significantly prolonged. However,ERα-IR were not significantly different in the ovaries of the female voles in the experimental and control groups,and the number of ERα-IRs in the bed nucleus of the stria terminalis (BST),the medial amygdaloid nucleus (ME),the medial preoptic area (MPO) and the ventromedial hypothalamic (VMH),in the experimental group were significantly less than those in the control group. There was no difference in the arcuate nucleus (ACR). In summary,zedoary glucose injection might affect reproductive behavior of female mandarin voles through changes in the number of the estrogen receptors α (ERα) in relevant brain regions.
摘要: To investigate the effects of the Male Sterile Preparation (MSP-1),an alcoholic male chemosterilant,on sperm count and sperm motility of the male plateau pika,40 adult male plateau pikas were randomly divided into 4 groups (n=10/group) that received 0,20,40 or 60 mg/kg bw of MSP-1 (ig). The weight of body,testis,epididymis and the count,motility,malformation rate of sperm were analyzed after 19 days. The body,testis and epididymis weights were not significantly different (P>0.05) at any dose levels when compared with the control group. Sperm count,motility and survival rate were significantly decreased in the treated groups compared with the control group. The sperm deformity rates in the 40 mg/kg and 20 mg/kg groups were higher in than the control group. The sperm deformity rates in the 60 mg/kg group were significantly higher than in the control group with a significant difference (P<0.01). MSP-1 was produced sterility in plateau pika by reducing sperm count,motility,and increasing the rate of sperm deformity.