摘要: Hypoxia induced factor 1 plays a key role during the cell hypoxia tran sduction Hypoxia induced factor 1α (HIF 1α) is a functional subunit of hyp oxi a induced factor 1 Plateau pika ( Ochotona curzoniae ),which is a Qinghai Tibet plateau native animal lived above 3 000 m,has high ratio of oxygen util ization to adapt to plateau hypoxia environments One fragment of the coding region of cDN A sequence of plateau pika HIF 1α was obtained by RT PCR technique using a deg en eracy PCR primer based on previously reported cDNA sequence in human, cattle, ho use mouse and Norway rat HIF 1α gene It was directly inserted into the vecto r pMD18 T DNA sequencing proved that it was highly homology with human (91%), c at tle (91%), house mouse (89%) and Norway rat (89%) HIF 1α gene The study prov id es basic important information for the HIF 1α cDNA whole sequence cloning of p lateau pika and its functional study
摘要: The contents and distributions of six unessential elements were analyzed in the whole bone, cranium, backbone, and leg bone of plateau zokor ( Myospalax baileyi ) and plateau pika ( Ochotona curzoniae ). These trace elements are Pb, Cd, As, Sb, Bi, and Hg. The results indicated that the contents of Pb, Cd, As, Bi and Hg in skeleton of plateau zokor are higher than that in skeleton of plateau pika with significant differences in Pb, Bi, and Hg. The contents of Hg and Cd between plateau zokor and plateau pika are significantly correlated while the contents of Sb and Bi are less correlated. In the skeletons of plateau zokor and plateau pika, the distributions of Hg, Pb, As, and Bi are mostly in the head bones, leg bones, backbones, and backbones, respectively. The contents of these six unessential trace elements in skeletons are related to not only to their ecological environments and food but also the cooperation and antagonism among these elements.