摘要: In 1999, we observed six Père David's deer calves (3 male and 3 female), which were born in the Beijing Milu Park to study the synchronization of action and grouping behavior in those calves. The observation began in the second week of their births until their weaning, altogether, we collected 4 320 behavioral records. We established two indexes, the Index of Activity Synchrony, A, and Daily Frequency of Calves in the Same Cohort, P, for measuring aggregate behaviour in calves. Before their weaning, the calves synchronised their activity (A=0.87±0.006), and they stayed in same cohort most of the time (P=0.86±0.012). No sexual segregation behaviour was found in the calves. There were no significant differences of A (F=0.075, P=0.788, df=1) and P (F=0.489, P=0.497, df=1) in the calves of the same sex or between opposite sex. However, Index A and P changed significantly as the ages of the calves increased. Daily frequency of calves stayed in the same cohort could not present aggregation tendency of calves exactly. At 4 week old, calves expressed the highest activity synchronization.

摘要: 本研究针对东北梅花鹿S100A4基因筛选出若干条RNAi靶位点,并利用NCBI中的BLAST工具去除脱靶情况,最终得到2条高分靶位点。然后在T4连接酶的作用下,将其与载体质粒pLVTHM的酶切产物进行连接,并通过PCR鉴定及测序筛选出阳性质粒。pLVTHM阳性重组质粒与pMD2.G及pCMV-dr8.91共转染到293T细胞中。在倒置荧光显微镜下观察转染效果并进行收获病毒质粒。结果显示在转染24h后,在293T细胞中观察到了绿色荧光。本研究成功构建出针对梅花鹿S100A4基因的慢病毒载体,为以后进一步研究S100A4基因在鹿茸再生中的具体功能与机制打下了基础。

摘要: 2009年1～12月,我们对宁夏贺兰山国家级自然保护区内的马鹿分布情况进行了调查。根据107个马鹿活动位点和572个马鹿痕迹位点,分别用95%的固定核空间法(Fixed kernel estimation,FKE)和100%的最小凸多边形法(Minimum convex polygon,MCP)进行计算,结果显示保护区内的马鹿分布面积约为642.33km2,占保护区总面积的33.19%,分布范围介于小水沟和榆树沟之间,插旗口和大水沟是马鹿分布密度最大的两个区域,而人为干扰较严重的黄旗口和小口子在调查过程中没有发现马鹿实体。我们对马鹿在冬季的种群数量进行了调查,利用模型均匀分布和简易多项式级数展开(Uniform+Simple polynomial),Distance 6.0的结果显示宁夏贺兰山马鹿的种群数量约为1420头(880～2300)。保护区内的马鹿在不同的季节有垂直迁移现象,冬季活动在低海拔地区。保护区内的马鹿集群较小(3.50±2.76,1～13头,n=107),且集群大小有季节性差异,冬季(4.26±3.09)明显大于其它季节。

摘要: 了解野生动物栖息地选择对于制定合理的物种保护和栖息地管理计划是十分必要的。2005年12月至2006年3月及2006年7～9月,在唐家河自然保护区,我们使用样带法和粪便法调查了毛冠鹿的冬夏两季栖息地选择情况,结果表明,毛冠鹿具有季节性的在高、低坡位进行迁移的现象,冬季毛冠鹿多出现在下坡位,而夏季多出现在中坡位和上坡位。同时,我们也发现冬季毛冠鹿多在距离道路较近的地方活动,而夏季毛冠鹿多在距离道路较远的地方活动。

摘要: 应用RT-PCR方法对海南坡鹿细胞分裂周期蛋白42(Cell division cycle 42,CDC42)编码区进行扩增,将扩增产物与PET-42a载体连接,重组质粒鉴定正确后,进行生物信息学分析;构建pET42a-hdCDC42表达载体,经IPTG诱导表达,将表达产物进行SDS-PAGE、可溶性分析、纯化及Western blot分析。结果表明,海南坡鹿CDC42含有1个576 bp的开放阅读框,编码191个氨基酸。经IPTG诱导表达后,得到一个带有His-tag和GST的约54 kD的融合蛋白,用抗His单克隆抗体进行Western blot,得到一条约54 kD的特异性抗体结合带,表明海南坡鹿CDC42原核表达载体成功构建并表达。