摘要: ＥＦＦＥＣＴＳＯＦＨＹＰＯＸＩＡＯＮＬＩＶＥＲＥＮＤＯＰＬＡＳＭＩＣＲＥＴＩＣＵＬＵＭＣａ２＋ＰＵＭＰＯＦＲＡＴ关键词低氧Ｃａ２＋泵高原鼠兔内质网肌浆网ＫｅｙｗｏｒｄｓＨｙｐｏｘｉａ，Ｃａ２＋ｐｕｍｐ，Ｏｃｈｏｔｏｎａｃｕｒｚｏｎｉａｅ，Ｅｎｄｏ...

摘要: 本文探讨了冷驯化（５℃）和脱冷驯化（２３℃）对高原鼠兔代谢产热的影响。在冷驯化中，鼠 兔的静止代谢率（ＲＭＲ）逐渐增加，血清Ｔ３／Ｔ４比率上升，肝线粒体状态３呼吸明显增加，线粒 体细胞色素Ｃ氧化酶激活，而肝脏线粒体蛋白含量没有明显变化、在脱冷驯化中，鼠兔的ＲＭＲ明 显降低，血清 Ｔ３／Ｔ４比率下降。表明冷激活肝线粒体呼吸（通过 Ｔ３／Ｔ４调控）是 ＲＭＲ增加的细胞学机制之一。鼠兔的非颤抖性产热（ＮＳＴ），褐色脂肪组织（ＢＡＴ）线粒体蛋白含量在冷驯化中明显增加，细胞色素Ｃ氧化酶活力显著升高；而在脱冷过程中，ＮＳＴ和ＢＡＴ线粒体蛋白含量明显下 降，表明ＢＡＴ是冷驯化中形成ＮＳＴ的重要器官。高原鼠兔通过ＲＭＲ与ＮＳＴ的调节，维持恒定 的体温，是对高原地区气温变化的适应性对策之一。

摘要: 1997～ 2 0 0 0年在青海省果洛州达日县 ,通过测定不同程度退化草地、人工草地和除杂草地中高原鼠兔种群数量的变化以及对草地的破坏程度 ,分析了草地植物群落变化对高原鼠兔种群数量的影响。结果表明 :随草地退化程度的加重 ,高原鼠兔种群数量相应升高 ,重度退化草地中高原鼠兔种群数量降低 ,是由食物资源不足引起。不同程度退化草地之间高原鼠兔种群数量差异极显著 (t未退化与轻度 =2 5 36 97,t未退化与中度 =2 5 5 5 ,t未退化与重度 =36 4 0 6 0 ,t轻度与中度 =2 3 2 794 ,t轻度与重度 =14 3439,t中度与重度 =2 0 1785 ,df =3,p<0 0 0 1)。高原鼠兔对草地的破坏面积与平均单坑面积呈显著的相关关系 (F =2 2 0 4 6 ,df=3,P <0 0 0 1)。在草地达到重度退化以前 ,高原鼠兔的种群密度升高 ,对草地的危害则加重。草地植物群落空间结构的变化直接影响高原鼠兔的种群数量 ,当植物群落高度增加时 ,其种群数量开始减少 ,反之则增加。

摘要: Hypoxia induced factor 1 plays a key role during the cell hypoxia tran sduction Hypoxia induced factor 1α (HIF 1α) is a functional subunit of hyp oxi a induced factor 1 Plateau pika ( Ochotona curzoniae ),which is a Qinghai Tibet plateau native animal lived above 3 000 m,has high ratio of oxygen util ization to adapt to plateau hypoxia environments One fragment of the coding region of cDN A sequence of plateau pika HIF 1α was obtained by RT PCR technique using a deg en eracy PCR primer based on previously reported cDNA sequence in human, cattle, ho use mouse and Norway rat HIF 1α gene It was directly inserted into the vecto r pMD18 T DNA sequencing proved that it was highly homology with human (91%), c at tle (91%), house mouse (89%) and Norway rat (89%) HIF 1α gene The study prov id es basic important information for the HIF 1α cDNA whole sequence cloning of p lateau pika and its functional study

摘要: The contents and distributions of six unessential elements were analyzed in the whole bone, cranium, backbone, and leg bone of plateau zokor ( Myospalax baileyi ) and plateau pika ( Ochotona curzoniae ). These trace elements are Pb, Cd, As, Sb, Bi, and Hg. The results indicated that the contents of Pb, Cd, As, Bi and Hg in skeleton of plateau zokor are higher than that in skeleton of plateau pika with significant differences in Pb, Bi, and Hg. The contents of Hg and Cd between plateau zokor and plateau pika are significantly correlated while the contents of Sb and Bi are less correlated. In the skeletons of plateau zokor and plateau pika, the distributions of Hg, Pb, As, and Bi are mostly in the head bones, leg bones, backbones, and backbones, respectively. The contents of these six unessential trace elements in skeletons are related to not only to their ecological environments and food but also the cooperation and antagonism among these elements.