摘要: 本文就蚕豆上潜蝇姬小蜂白昼活动规律进行了研究，在午间１３：００－１４：００时气温最高时，为潜蝇姬小蜂的活动高峰期，种群（Ｙ）和温度（Ｘ）的白昼活动模式为 Ｙ＝－２．４１＋１． ３８ｘ－ ０．０９ｘ２， ｒ＝ ０．７２２１；在蚕豆成熟时，潜蝇姬小蜂种群数量最大。

摘要: By using the domestic silkworm as the bioreactor (reorganizing the nucleus polygon virus expression system) to produce genetic products, we can attain our goal with less cost, higher productivity and activity in a safe manner. With the swelling development of biosciences and biotechnologies in recent years, we should develop new experimental species, especially the lower species, to replace the mammals and explore the animals' potential as a resource to benefit the human beings. We will make a detailed account of what we have achieved by utilizing the domestic silkworm exotic genes and the progress we have made in its animalization breeding technologies. Our domestic silkworm expression system is composed of its cells, transferring carrier, modified BmNPV, its chrysalis. We have obtained the rsj14NPV, which contains Japanese schistosome fatty acid albumen genes, and rsj28NPV, which contains Japanese schistosome 8KDGST genes. After being infected by the rsj14NPV, every silkworm (871×872) can produce 3.5 mgs' purified albumen. After the silkworm 57A.57B×24.26 is infected with Rsj28NPV, every silkworm will turn out 5mgs' purified albumen. By employing rSj14NPV(871×872),we observe the relations between the volume of contracted virus, the timing of the contraction, the duration of the virus and the amount of the produced albumen. The results are as follows: the silkworm that is inoculated with 4μL and 6μL produces 4-5mgs/silkworm, which is the highest, while the silkworm that is inoculated with 1μL group and 2μL group turns out only 1 mg, which is the lowest; the timing of the inoculation has no significant influence on the productivity; the reorganized virus that is preserved for a long period of time(9 months)is not very productive after being inoculated, which shows that we should use the required resurrected reorganized virus to achieve high productivity; by inoculating 55μLs’ resurrected reorganized virus(rsj14NPV) into the silkworm 871×872 will produce 4-5 mgs' albumen, which is similar to that of autumn wind ×white jade. In accordance with the current know how of sericiculture, we establish the common level silkworm breeding technical system, and set down the corresponding technical rules and disease tackling principles. The common level silkworm breeding technical system is composed of 6 technical links, that is, hatching hastening of the eggs; the picking and storing of mulberry leaves; breeding criteria of the 1 3 instar young silkworms; breeding criteria of the 4 5 instar silkworms; driving the silkworm onto a pile of straws to spin; antisepsis and preventing illness. We have confected the man made feeding stuffs for silkworms of all instars and the research on the feeding of the silkworm barrier system silkworm is still in progress. In the research, we will establish a technical system that can control the breeding environments and avoid pathogenic pollutions and the use of antibiotics. It can also carry out recycling breeding so as to meet the needs of silkworms of all kinds.

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摘要: 【目的】鉴定家蚕Bombyx mori马氏管特异表达基因及其启动子，为家蚕的代谢排泄、解毒及免疫相关基因功能的研究及利用提供新的工具与支撑。【方法】基于家蚕全组织蛋白质组学数据进行马氏管特异表达基因筛选并利用RT-PCR验证基因在家蚕5龄幼虫头、表皮、中肠、脂肪体、马氏管、丝腺、精巢、卵巢、血细胞及气管中的表达；利用qRT-PCR检测家蚕不同发育阶段(卵、 3龄眠、 4龄幼虫、 5龄幼虫、上簇期、蛹和成虫)马氏管中及5龄幼虫头、表皮、中肠、脂肪体、马氏管、丝腺、精巢、卵巢、血细胞和气管中上述特异表达基因的表达量；利用PCR克隆家蚕马氏管特异表达基因BmPiT4175的上游启动子序列，并构建以EGFP为报告基因的转基因载体，获得转基因家蚕，在个体水平验证BmPiT4175上游启动子的活性及组织特异性。【结果】筛选获得了在家蚕马氏管特异表达的基因BmPiT4175;所克隆的BmPiT4175启动子长2 955 bp,其驱动的EGFP仅在转基因家蚕马氏管中特异表达，且EGFP与内源基因BmPiT4175的表达量相当。【结论】BmPiT4175的表达模式及其启动子活性都是家蚕马氏管特异的。

摘要: 本文从蚕桑生产情况、加工流通、消费趋势、进出口市场和贸易变化、全产业链效益以及产业政策等方面阐述了2022～2024年我国蚕桑产业发展态势，总结了蚕桑产业发展的主要特征包括传统蚕桑生产稳中有进、新品种新技术应用加速、蚕种生产向优势区域和企业集中；丝绸加工量减效增、食品加工多元发展、药品加工冷暖不均；消费方面丝绸服装消费回暖、蚕丝被消费增长明显、多元化消费转型以及文旅融合发展加速，但传统丝绸商品进出口减少，蚕种出口呈逐年增长态势；全产业链效益方面蚕茧、生丝价格持续上涨。认为我国将长期保持世界第一的蚕桑产业地位，产业比较经济效益长期向好，蚕种供给是未来产业发展的重要制约因素，并从坚持高端引领，编制“十五五”蚕桑产业发展规划；推动种业创新，全力提升蚕种生产能力；坚持集成创新，重点提升现代装备水平；维护市场秩序，着力提升优质蚕茧产量；发展新型业态，多元拓展资源综合利用；强化技术支撑，优化产业技术服务体系等方面提出了相应的对策建议。