摘要: In order to investigate the effect of zedoary glucose injection on reproductive behavior in female mandarin voles(Microtus mandarinus),behavior focal sampling and immunohistochemistry methods were used to examine the relationships between behavior changes of female mandarin voles and estrogen receptor α immunoreactive neurons (ERα-IRs) in the ovary and in relevant brain regions after being fed with rabbit chow that was mixed with concentrations of 30 mg/kg zedoary glucose injection. Healthy adult female voles were divided into experimental and control groups. The experimental group was fed with the mixed chow and carrot,and control group was fed with rabbit chow and carrot. Behaviors of the adult female voles were observed after being fed for 30 days. The numbers of litters of these adult female voles after mating with adult male voles was recorded,and meanwhile the number of ERα-IRs in the relevant brain regions was detected. The results showed that:during the period of intraspecies female-male interactions in a cage,the female voles in the experimental group displayed more escape behaviors,defensive behaviors,and hostile attacks than did those in the control group. In addition,the number of litters born to the female voles in the experimental group was significantly less than to the control group,and the incubation period of females in the experimental group was significantly prolonged. However,ERα-IR were not significantly different in the ovaries of the female voles in the experimental and control groups,and the number of ERα-IRs in the bed nucleus of the stria terminalis (BST),the medial amygdaloid nucleus (ME),the medial preoptic area (MPO) and the ventromedial hypothalamic (VMH),in the experimental group were significantly less than those in the control group. There was no difference in the arcuate nucleus (ACR). In summary,zedoary glucose injection might affect reproductive behavior of female mandarin voles through changes in the number of the estrogen receptors α (ERα) in relevant brain regions.

摘要: 小型哺乳动物能够通过行为策略的进化适应周围的环境。其中,种内社会组织也可随环境参数的变化而变化。河南灵宝程村气候干燥、海拔高,而新郑则气候湿润、海拔低。来自河南灵宝程村的棕色田鼠种群和来自新郑的种群是否在社会行为、情绪、以及性二型上有所差别,目前还不清楚。本研究中,我们通过旷场实验和熟悉选择试验比较了两个种群的情绪、运动能力以及对熟悉鼠和陌生鼠的选择。统计结果表明,程村种群的雌性个体比雄性个体重,而新郑种群的雄性体重比程村种群的重。在旷场实验中,程村种群比新郑种群表现出较多的焦虑样行为。配偶选择实验中,新郑种群不论雌雄都表现出了对陌生异性的喜好。程村种群雄性则更愿意选择熟悉的个体。而且,程村的雌性待在陌生箱攻击陌生鼠的时间远大于熟悉鼠。以上结果表明,两个野生种群在体重、情绪以及对熟悉鼠和陌生鼠选择上都表现出了明显的种群间差异。

摘要: The plasmid cDNA library from the liver of Microtus fortis was constructed by using SMART technology. The titers of this cDNA library were 1076 pfu /μL,the recombination rate was about 94%,the library capacity was 1.08×106. 22 cDNAs chosen at random were sequenced. After BLAST analysis of these sequences,it was found that these cDNAs showed more than 75% similarity to the functional known genes of mice,rats and humans in the Genbank. The cDNA library was very important to the research of fatty liver-related gene screening and clones. These provided a base for further study on the molecular biology,fatty liver model development and pathogenesis research of Microtus fortis.

摘要: 通过观察产后2日(L2)、6日(L6)、10日(L10)、14日(L14)和20日(L20)的棕色田鼠母鼠对2日龄、6日龄、10日龄和14日龄非亲缘幼仔的攻击行为和衔回行为,探讨了哺乳期棕色田鼠的母性行为与非亲缘幼仔的发育(日龄或个体大小)及母鼠产后时间的关系。结果表明,在哺乳前期(L2,L6),非亲缘幼仔的日龄大小显著影响母鼠的攻击行为(P<0.05);在哺乳中期(L10,L14)及后期(L20),非亲缘幼仔的日龄对母鼠的攻击行为没有显著影响(P>0.05)。在整个哺乳期,母鼠对14日龄非亲缘幼仔的攻击频次最高(P<0.05)。产后不同时间的母鼠对同一日龄非亲缘幼仔的攻击频次没有显著差异(P>0.05)。这些结果表明,哺乳期内的棕色田鼠对非亲缘幼仔的攻击与幼仔的发育有关,与母鼠的产后时间无关。

摘要: 采用强制给药和自由取食2种方式,研究了雷公藤制剂对发育期成体雄性布氏田鼠不育作用,给药剂量分别为80mg/kg、120mg/kg和160mg/kg。结果表明,120mg/kg和160mg/kg剂量使睾丸脏器系数下降,但剂量组间差异不明显(F(3,21)=2.197,F0.05=3.07,P>0.05)。不同剂量能够对附睾中精子数量和活力产生显著影响(F(3,21)=17.305,F0.05=3.07,P<0.05),且随药物剂量的升高精子密度与精子活力均呈下降趋势。精子畸形率亦随药物剂量的增加而增加。80mg/kg及其更高的剂量使附睾出现萎缩并造成睾丸组织损伤。120mg/kg剂量可减少布氏田鼠平均胎仔数及降低繁殖率。给药后的繁殖后代未发现畸形幼仔。雷公藤制剂的最佳使用剂量介于120～160mg/kg。自由取食与连续强制给药两种方式对雄性布氏田鼠的作用差异不显著。