摘要: Being widely distributed in the Qinling Mountains,Bashania fargesii is the primary food source for giant pandas (Ailuropoda melanoleuca) during winter and spring.To understand the relationship between panda food habits and nutrients in the bamboo,we collected samples of leaves,branches and stems across seasons and ages in Foping Nature Reserve,Shaanxi Province,China. Each category of samples was collected at the same site,with three replicates,and weighing 200 to 500 gram. Seven trace elements (Zn,Cu,Fe,Mn,Ca,Mg,K) were analyzed by the atomic absorption spectrophometric method. Crude protein was determined following Macro-Kjelhahl procudure,and crude fat was measured with Soxhlet procedure. The results indicated that contents of Mn,Ca,Mg in the leaves and Cu,Zn,Fe in the branches were respectively higher than those in the other parts on bamboo. Compared with those in the branches and stems,contents of crude protein and crude fat in the leaves were significantly higher. In addition,contents of trace elements and nutrients in the bamboos varied across seasons and ages. Leaves are the most nutritious among different parts of bamboo. Food habits exhibited by giant pandas were closely related to nutrition quality of leaves in the bamboo.
摘要: Scleroderma is a connective tissue disease characterized by the fibrosisization of skin and internal organs.In this study,the genomic sequence and cDNA of Sjogren's syndrome/scleroderma autoantigen 1(SSSCA1)gene of the giant panda was cloned successfully using RT-PCR.The cDNA fragment cloned was 642 bp in length,and contained an open reading frame (ORF) of 600 bp encoding 199 amino acids.The genomic sequence was 1 262 bp,containing four exons and three introns. The deduced amino acid sequence showed that this protein was composed of 199 amino acids and its estimated molecular weight was 21.53841 kDa with a pI of 5.14. Three different patterns of functional sites were found:one protein kinase C phosphorylation site,five casein kinase Ⅱ phosphorylation sites,and three N-myristoylation sites. The SSSCA1 gene of the giant panda were highly homologous to those of some other mammals.
摘要: Based on bitter taste receptor T2R2 gene sequence of domesticated dog(AB249685), one pair of primers were designed and used to amplify an approximately 1.1 kb DNA fragment from genomic DNA sample of giant panda by using PCR. The PCR products were ligated into the pMD-18T vector, and then transformed into competent cells of E.coli DH5α. The identified positive clone was sequenced. The result showed that the T2R2 gene of giant panda was 1 008 bp in length, and contained complete exon, and 915 bp, encoding 304 amino acid residues. The pI of the protein is 9.56 and its molecular weight is 34.90 kDa. The prediction of topological structure for the protein indicated that it contained 11 potential functional sites(three N-glycosylation sites, three potential protein kinase C phosphorylation sites, two N-myristoylation sites, one Casein kinase II phosphorylation site, one cAMP- and cGMP-dependent protein kinase phosphorylation site and one Nuclear Localization Signal site) with five sites observed only in giant panda, and the protein comprised seven transmembrane helix regions, and four extracellular regions and four intracellular regions. The T2R2 was a hydrophobic protein with less hydrophilic components which mostly were located on the intracellular regions. Alignment analysis revealed that the homology of T2R2 gene nucleotide sequence of giant panda with that of dog, cat, cattle, horse, chimpanzee and mouse is 92.65%, 91.12%, 85.64%, 86.73%, 85.20%, 72.59%, respectively, and the homologies of amino acid sequence is 86.73%, 85.20%, 74.67%, 78.62%, 75.66%, 60.53%, respectively. On the whole, the giant panda T2R2 gene was high evolutionarily conserved, but its protein presented more abundant functional sites than did those of other species. However, the correlation between the characteristics of T2R2 gene and giant panda’s special diet needs to be further studied. The obtained sequences were submitted to GenBank, with accession NO.FJ812726.