摘要: 目的　研究日粮高钙含量时 ,实验比格犬对钙磷肠吸收的影响。方法　消化试验。结果　当日粮含钙量从1 2 %提高到 2 4 %时 ,粪钙排泻量明显增加 (P <0 0 5 ) ,钙表观消化率逐渐降低 ,肠钙吸收量增加。随日粮钙含量提高 ,粪磷排泻量明显增加 (P <0 0 5 ) ,磷表观消化率和肠磷吸收量明显下降 (P <0 0 5 )。结论　犬主要通过改变肠钙排泄量来调节机体对钙的吸收 ,此外 ,高钙抑制消化道对磷的吸收

摘要: 通过分组测量山东细犬体重、颅长、鼻长、颈长、前肢长、后肢长、尾长、胸围、腰围、体宽、身高、体长 ,并观察牙齿个数、毛色、眼色和性格 ,报道山东细犬形态结构数据 ,并制定了山东细犬形态学标准

摘要: 能量是营养学的重要指标 ,能量摄入和消耗之间的失衡对动物的健康及保持作为实验动物的重要特性都会产生影响。对于犬的能量需要研究一般是研究基础代谢率 ,近年来研究能量需要较多 ,比如热量测定法或保持体重恒定法。保持体重恒定法是指一定时间 (5周以上 )保持体重恒定时的能量需要为主要指标 ,保持体重恒定时的进食能量即为能量需要。通过选取 1岁左右健康山东细犬 12只 ,随机分为 3组 ,每组 4只 ,各组分别喂不同能量水平的日粮 (能量水平分别为 10 2 2MJ kg、11 2 3MJ kg、11 92MJ kg) ,每天上午定时定量饲喂 ,自由饮水。每周空腹称重。饲养试验的第 15d开始收集粪便 ,连续 4d ,将 4d粪便混匀在一起 ,部分取样进行水分和含氮量测定 ,其余部分制成风干标本测定能量 ,用测热法测定能量。主要结果如下 :三组动物在不同能量水平饲喂 6周体重增加不明显(P >0 0 5 ) ;三组动物粪能和尿能无显著性差异 ,但消化能和代谢能差异显著 (P <0 0 5 )。说明对于山东细犬 ,维持体重的恒定不需要刻意控制饲料的能量水平 ,其自身可以通过不同的代谢水平调节。

摘要: Guangdong Beagle Resource Research and Development Center (abbreviated as GBRDC ) was Founded by Guangzhou Pharmaceutical Industry Research Institute in 1983, it is named as GBRDC by Guangdong Province Sci. & Tech. Commission. It is then first Beagle Dag Research Resources according to Laboratory Animal Science standard to maintain the Beagle Dogs research resource in China. Currently, it is playing a leading role in Beagle Dog research resource in China. During the 20 past years, GBRDC's Management, Technology, Research are all focused on the regulatory testing and animal welfare. 1. Management: Facility Profile, Environmental Conditions;Colony Management;Pedigree (Family ) Files. 2. Technology: Preventive Medicine Program;Microbiological Diagnostics and Testing;Animal Clinical Medicine and Pathology Lab;Genetic Quality Control. 3.Research: Beagle Genomic Research Program;Beagle Cell lines and ES Cell Culture;Beagle behavior training program. First successed using in Customs Quarantine.

摘要: With small somatotype, gentle temperament, balanced hematological and blood biochemical index, Beagle was ideal for laboratory use. Since 1980, beagle has been introducted into our country. They were successfully bred and multiplied in Guangdong , Shanghai and Beijing . But the control of heredity, balance of gene frequency, gene diversity and gene differentiation and protection of idioplasm has not been well studied. Falk & Holsinger(1991)point out: we can not protect what were unknowed. For operative preventing the inbreeding diminution , gene segregation and differentiation, the DFP and randomly amplified polymorphic (RAPD) technique were applied to assess the genetic variation and colony heredity structural analysis among the population of three internal beagle centers and Marshell beagle in America. The experimental exponent of the heredity analysis in different colony were from Guangdong, Shanghai, Beijing and Marshell (Amereca), and they were not relative. The experimental exponent of the different generation were from Beijing colony, and they were direct maternal side in different generation, but each individual were no relative in the same generation. The heredity structive relatio on different colony and internal colony were analyzed with Phyltools6.0 and SAS6.12 software. The dendrogram of UPGMA based on Nei's genetic identity of four colony wre constructed. The result showed: There were average distinguished bands 9.8±1.789 in America colony, 10.2±0.837 in Guangdong colony, 10 8±1 483 in Shanghai colony,11.2±2.280 in Beijing colony,and there were 10.5 average bands in the four colonies. The bands owned by both were 0.204 in America colony, 0.294 in Guangdong colony, 0.370 in Shanghai and 0.267 in Beijing respectively. The similarity coefficient were 0.508—0.557(average 0.526). there were no marked difference in the four different area colony. The dendrogram of UPGMA based on Nei's genetic distance coefficient was drawn: the gene distance of colony between Guangdong and Shanghai was close, and the gene distance of colony between Beijing and America was close. The heredity differentiation and isolation were analyzed with the formula of coefficient of gene dedifferentiation[g=(dt-ds)/dt. Dt was the average partnership distance of all the individuals. Ds was the average partnership distance in the group.].The result showed: the coefficient of heredity dedifferentiation in Marshell was 0.02, the coefficient of gene dedifferentiation in Guanddongwas 0.02, in Shanghai was 0.02, in Beijing was 0.02. It showed the heredity dedifferentiation and isolation among the closed groups were formed. Because the DFP technique was used on genome DNA intron, the RAPD technique was used on genome DNA exon. The result showed: the average smililarity coefficient in different colony was 0.859—0.898. the smililarity coefficient was 0.859±0.0299 in America colony.0.888±0.0315 in Guangdong, 0.898±0.0261in Shanghai,0.884±0.0330in Beijing respectively. The DFP techque was applied to assess the genetic status in Beijing beagle colony. The result showed: the smililarity coefficient was 0.540±0.091 between F1-F2, 0.493±0.128 betweenF2-F3, 0.481±0.100 betweenF1-F3(average 0.505).and F1-F2>F2-F3> F1-F3 .The smililarity coefficient was 0.577±0.124 in F1,0.550±0 009 in F2,0.469±0.113 in F3. Using Sas Analysis of Variance Procedure, there was marked difference between F1 and F2, but there was marked difference between F2 and F3, between F1 and F3. Using the derivation formula of coefficient of inbreeding, the coefficient of inbreeding was 0.334±0.167 in F1, 0.355±0.111 in F2, 0.227±0.100 in F3. The result indicated that the multiply of the three generation was unfit for the themry of Hardy Weinberg Conclusion: 1.Extreme geography differentiation and isolation have been formed in different laboratory beagle groups; 2.There are abundant genetic diversity in the population. 3. The primary beagle closed populations have formed in China on the base of genetic analyse. 4. Severe genetic diversity in idioplasm has been f