摘要: 昆虫病原线虫储存困难是目前应用线虫作为生物杀虫剂的一大障碍，本文比较了不同温度、不同储备方法、载体不同湿度对线虫存活和质量的影响，为大田应用提供参考。结果表明，温度与线虫的存活、毒力呈负相关。载体的湿度与线虫的存活呈正相关。若洗净线虫，载体有９０％以上的含水量，线虫在较高的温度下（２８～３０℃）仍能存活１５～２５天，且质量较好。

摘要: 本文根据系统分析方法，利用正交旋转组合设计于固体培养系统中测定培养基的装样量、细菌培养时间、接线虫量和线虫培养时间对Ｓｔｅｉｎｅｒｎｅｍａｃａｒｐｏｃａｐｓａｅ和Ｈｅｔｅｒｏｒｈａｂｄｉｔｉｓｂａｃｔｅｒｉｏｐｈｏｒａ产量的综合影响，确定线虫的优化培养参数。在设计组合范围内，接线虫量对Ａ２４线虫产量的影响显著；培养基的量和细菌培养时间对线虫产量的影响不显著。对Ｈ０６线虫产量，接线虫量、培养时间呈显著的正影响；细菌培养时间则呈显著的负影响。此外，接线虫量与培养时间对两种线虫均产生明显的负交互效应。培养基的装样量对线虫产量无显著影响。通过频数分析，分别得到了Ａ２４和Ｈ０６线虫的培养参数的优化组合。即每克培养基的Ａ２４线虫产量大于５０×１０４时，培养参数的优化组合是培养基的量为每瓶５３．６～５８．８克，细菌培养时间２天左右，接线虫量约每克培养基４．６×１０３，培养时间为１６～１８天。同理，每克培养基的Ｈ０６线虫产量大于２５×１０４时，培养参数的优化组合分别为每瓶培养基的量６７．８～７４．２克，细菌培养时间１～２天，接线虫量为每克培养基５．５×１０２～２．２×１０３之间，培养时间２２～２４天。根据这些培养参数的优化

摘要: 本文报道了昆虫病原线虫对白杨透翅蛾控制作用的研究。结果表明，小卷蛾线虫的Ａｇｒｉ－ｏｔｏｓ和Ｂｅｉｊｉｎｇ品系对白杨透翅蛾的侵染力较强。通过毒力测定，两个品系对２龄幼虫的ＬＤ５０值分别为２４条／头和２７条／头，对４龄幼虫的ＬＤ５０值分别为１２条／头和１３条／头，两个品系毒力差异不显著。在寄生过程中，Ａｇｒｉｏｔｏｓ品系与白杨透翅蛾幼虫仅接触０．５小时，每虫就可被侵入１．２条线虫，１２小时基本可达寄生高峰，２４小时寄生的死亡率为１００％。可见，接种时间越长，寄生线虫数越多，死亡率越高，死亡速度越快。Ａｇｒｉｏｔｏｓ品系的寄生性和致病性均强，是防治白杨透翅蛾很有希望的生物因子。Ａｇｒｉｏｔｏｓ和Ｂｅｉｊｉｎｇ品系在室内以注射法防治虫瘿中的幼虫效果较好，４８小时防治效果可达９０％以上，而且剂量越大，效果越好，以１５０条线虫／亳升为宜。两个品系以１０００条线虫／毫升进行林间防治，效果均优于敌敌畏。对主干和成树上的幼虫防治效果好于枝条。

摘要: With the development of science and technology, life science is involving in many fields. The requirements for the methods of experiment and experiment object are more abroad, more strict and more diversified. As laboratory animals of experimental material in the life science, great changes have taken place since then. As for the exploitation of new kind of laboratory animals, it is not only limited in experimental Animalization of ordinary mammals but also expands steadily from Experimental Animalization of birds, reptiles, fish and invertebrates. Now insects of invertebrate have been used in life science research and are enriched gradually in application fields. China is one of the earliest countries that exploits and makes use of insect resources in the world and is also the country with the largest insect resources on the earth. At the present, the insect resources are studied, developed and utilized in many fields and on all levels regarding the traditional raising as its foundation and the biological technology as the guide. The scope of insect research expands from insect body to the function, microorganism, and the use of insect gene, which is developed widely in the fields of agriculture, medicine and industry. In agriculture, the biologic pesticides have been researched, developed and utilized since 1960's in our country. The biological prevention of our country has ranked among the advanced countries. With the further development of research of biological prevention and cure, the experiment insects, acting as experiment material used in biologic pesticide, are playing important roles in pathogenic filtration virus's multiplication, measurement of toxicity and mould of insects etc. Therefore, the experiment insects' house raising, standardization of condition control, legalization and standardization of management, and socialization of production and supply are the developing trends of biologic prevention and cure and development of other fields of life sciences. According to the raising experience, existing conditions, human resources and some other groundwork of Wuhan la boratory animal center, we have made progress in the preliminary study of experiment insect's animalization which mainly involving in lepidopteron such as ,bollworm, beet noted, fleck noctuid . According to the requirement of standards of quality and environment of experiment insects of our country and combining the design and present conditions of our labortory animal building , we have improved the environment and facilities of the experimental insects room and have controlled reasonably the sunshine, temperature, humidity, ventilation , fitful feeding (cage) shelf and microbes surroundings in and out door, which are different for insects in their different phases of growth so as to make indoor environment to meet the breeding demands of experimental insects. On the basis of meeting the lab's needs of ordinary scientific research and development, we have mapped out stable condition monitoring standards for experiment insects. We have made efforts to map out other standards with regards to the research of artificial feeding stuff for lepidopteron; counting and measurement of nutrition percentage; improvement of technology relating to processing and storing, etc. so that these measures will ensure the experiment insect's high quality and normal reproduction. Presently, bollworm's continuous in door block out reproduction have reached thirty generations, while beet noctuid reached twenty five generations with stable capability of production and supply scale. We can supply ten thousand new bollworms for the experiment purpose per day. On the basis of standardizing the experimental condition and house raising, we will develop further the inspection experiment of physiological, biologic and chemical function index and hereditary feature to microbes and vermin of the experimental insects so as to map out a relevant monitoring quality standard for providing life sciences with sufficient theoretical basis. With the further development

摘要: 随着生物农药的不断发展,许多昆虫也逐步加入到实验动物行列中,甜菜夜蛾在农业及生命科学相关领域的研究中作为科研材料发挥着重要的作用。利用合理的人工配方饲料,通过室内长期的继代饲养繁育,确定了甜菜夜蛾生活史中各个阶段温度、湿度和光照等技术参数,掌握了甜菜夜蛾室内人工饲养管理的关键技术,从而建立了适宜甜菜夜蛾生长发育以及连续传代繁育的环境设施条件和技术方法。目前,甜菜夜蛾已经在室内连续传代超过100代,其产卵率、孵化率、幼虫成活率、化蛹率和蛹质量以及成虫羽化率等都一直处于稳定状态,种群未出现退化现象。