摘要: The hairless mouse strain was first found in KM mice. It has been bred to 19 th generation for the time being. The life span of the hairless mice was about 24months under grade2 breeding , To testify whether they represent a new mutation strain, we studied their biological characters macroscopically. We found 2 hairless mice (a male and a female respectively) in the sibling of KM mice initially. They were kept and bred by means of random, complete, brother sister inbreeding. Fertility was once reduced in 7 th 9 th generations, but it gradually returned to normal by the method of random, cousin inbreeding. It has confirmed that hair loss of the mice had no relations with aging, nutrition, mainteiteining condition, parasitization and sexuality. Female mice had ability to suckle their children till ablactation. Immature KM hairless mice (KM HM) had pale white fuzz when ablactated. It began to lose hair at day 30. Little residual fuzz could be found around the eyes and root of the tail at day 30 to 42. Then they kept hairless for the rest of lives. The skin of KM HM was smooth and clear, through spleens, could be seen. Some folds of the skin appeared and became obvious on the head and lateral part of the body with aging. KM HM had thymus at birth, whose size and weight were almost the same as the counterparts faith normal hairs. Under grade 2 circumstances, the growth and reproductive ability of KM HM had no difference with that of hairy KM mice. The offspring produced at one pregnancy was from 3 to 13. The mother KM HM had the ability to suckle inborn and occasionally ate her babies in the firstborn. Solid tumors were observed occasionally in the mature mice, but when transplanted to other mice, they could not grow well and gradually disappear, some even did not grow. They occur in two mature female mice at present. Both are located in the trunk. One is about 5 to 6mm apart from groin, the other about 2 to 3mm apart from right forearm. KM HM had thymus and could keep alive when in conventional condition, which were different from nude mice without thymus. The character of KM HM, that their folds on the skin became evident with time going on, was similar to that of reported Yuyi hairless mice and Rhinoceros mice. But hair loss of rhinoceros mice began at 12d after birth, and a few separated hair still existed until 60d. Occasional sporadic tumors, residual fuzz only present in Certain areas and the lactation ability in these KM HM mice seemed to distinguish them from the Yuyi hairless mice. All of above suggest that further investigations need to be continued.
摘要: The mutant gene of BALB/c mutant hairless mice was assigned to chromosome 11, Genetic markers which have been tested suggested that the mutation is a new genetic locus that affected the skin and hair structure of the mouse .The mutation was named uncoved,with the symbol Uncv . Uncv has been accepted by International Naming Committee of Mice,and the gene information of Uncv has been receipted by the mice's genebank .The skin is the biggest organ of the body , and is also the main physiological barrier between vivo and vitro .The skin is albe to produce and keep the part immunoresponses , inflammation . Many immunoresponses are related to the skin .The dissecting and breeding of the mutant mice show that their immune organs are normal and they can adopt to the general conditions.Objective To further study the mutant gene's immune function of the BALB/c mutant hairless mice . Method\ We tested the parameters of the immune system about two day old、two month old hairless mice and mutant sparse coat mice . After testing the parameters of CD4+ 、CD3 + 、CD8 + 、CD19 + through flow cytometry and testing the IgG by ELISA.Results\ we found that CD19 + 、CD4 +/CD8 + of male are higher than female about two week old mutant mice with sparse coat , but CD8 + of female is higher than the male,CD4 +/CD8 + of male F 2 sparse coat mice is higher than the female, CD19 + 、IgG of male mutant hairless mice is higher than the female . There were no significant differences among the male and the female about the two month old mutant hairless mice and the mutant sparse coat mice, CD4+ of male F 2 two month old mutant hairless mice is higher than the female . Otherwise , we also found that the celluar immunity and humoral immunity of both two week old and two month old hairless mice are lower than the mutant sparse coat mice ;only the celluar immunity of F 2 mutant hairless mice is lower than the mutant sparse coat mice , but the humoral immunity is higher .The experiment suggest that the mutation of mutant hairless mice can down regulate the mice's immunity .Our studies showed that the mutant gene of BALB/c hairless mice affected the immune function.