摘要: 目的研究微卫星DNA多态性在海南和广西恒河猴遗传多样性中的应用。方法利用11个微卫星位点,对来自这两个地区的恒河猴进行了遗传检测,并通过POPGEN32软件计算各个微卫星座位的等位基因频率、有效等位基因数目(Ne)、多态信息含量(PIC)和遗传杂合度(H)。结果所选择的11个微卫星位点均存在高度的遗传多态性,H为0.6848～0.8785;PIC为0.5826～0.8345;Ne为3.0000～7.1553。以11个微卫星标记为测度,广西恒河猴的Ne、PIC和H的平均值均高于海南猴,分别为4.2583、0.7090、0.7706(广西恒河猴)和4.2054、0.7025、0.7656(海南恒河猴)。结论这种差别可能与地域来源有关。这些研究为微卫星标记分析恒河猴遗传多样性提供理论基础。

摘要: Objective\ The purpose of this paper is to study the influence on monkey,s generative physiology after terminating early pregnancy by focused ultrasound beams. Methods\ Five early pregnancy Macaque monkeys were treated with focused ultrasound beams, observing the recovery of menstruation and the change of skin after treatment, which show the hormone level.Two circles after the coming of menstruation, we estimated the shape and size of uterus and determined if there are some injuries on adjacent organs , by color Doppler ultrasonography (CDFI). Results\ Menstruation of these Macaque monkeys came 40 days after being treated with focused ultrasound beams to terminate the pregnancy, the volume and lasting period were normal, CDFI shows that the size and shape of uterus were normal, the endometrial line was clear to be observed, and there are no injuries on adjacent organs. Conclusion\ it is safe to terminate an early pregnancy of monkey model by Focused Ultrasound Beams, and there is no influence on the generative physiology, it has a potential prospect of being applied in clinics.

摘要: 目的研究两种抗应激剂对恒河猴热应激的影响。方法将6只恒河猴随机分为A,B两组采血做9项血液学及9项血液生化指标测定,A组日粮中每只添加VC300 mg/d和VE50 mg/d;B组日粮中添加0.2%苜草素,饲喂两个月后分别将A、B两组恒河猴放入(32±1)℃,湿度70%猴房内,1 h后采血,测定相应血液学及血液生化指标。结果两组动物血液学各项指标试验前后差异不显著(P>0.05);而血液生化值呈现:A组试验后AST、ALP、LDH分别为(101±23.64)U/L,(623.67±233.12)U/L,(1384.33±807.66)U/L,试验前后差异不显著(P>0.05);B组试验后TP、ALB分别为(83.83±16.32)g/L,(38.53±0.57)g/L;试验前后差异不显著。结论VC和VE在恒河猴抗热应激过程中主要作用于维持血液中AST、ALP、LDH的活性稳定,而苜草素主要作用于维持血液中TP、ALB的稳定,两种添加剂能有效提高恒河猴抗热应激的能力。

摘要: 目的摸清B病毒(BV)感染现状,从而有效降低BV在恒河猴群中的感染率。方法采用ELISA方法对恒河猴血清进行抗体检测。结果检测样品629份,其中350份呈BV抗体阳性,10份血清呈BV抗体可疑,其余血清为抗体阴性。结论种猴(≥11周岁)BV感染率89%;青年猴(3～10周岁)BV感染率39.2%;幼龄猴(≤2周岁)BV感染率7.6%。随着年龄的增长,恒河猴群中BV感染率越高。

摘要: 目的本实验通过在幼年恒河猴日粮中添加不同浓度的苜草素,对实验前后组间IgG、IgA、IgM进行比较分析。方法将15只人工饲养的健康幼年恒河猴随机分为3组:A组为对照组,饲喂基础日粮;B、C组在基础日粮中分别加入0.1%和0.2%的苜草素。饲喂两个月后检测15只恒河猴血液中IgG、IgA、IgM水平。结果A、B、C三组实验后IgG分别为644.8 mg±72.23 mg/dL7、56.4 mg±55.87 mg/dL、776.4 mg±193.84 mg/dL;IgA分别为12.41 mg±3.31 mg/dL1、7.2 mg±1.77 mg/dL、19.75 mg±0.72 mg/dL;IgM分别为42.66 mg±4.45 mg/dL6、5.58 mg±10.11 mg/dL、71.36 mg±10.62 mg/dL。结论B、C组实验后IgGI、gAI、gM水平明显高于A组(P<0.05),同时两组实验后IgG、IgA、IgM水平也明显高于实验前(P<0.05),实验后C组IgG、IgA、IgM水平明显高于其他两组(P<0.05),表明苜草素能有效提高猕猴免疫球蛋白水平,增强猕猴自身免疫力。