摘要: 本研究成功地筛选了家蚕作为松毛虫狭颊寄蝇Carceliamatsukarehae的替代寄主。松毛虫狭颊寄蝇能产卵寄生于家蚕，并完成其生活史，子代生活力、繁殖力较强，1头家蚕幼虫可以繁殖1到2头蝇。同时，狭顿寄蝇偏好寄生于寄主的高龄幼虫。五龄眠起1～2天的家蚕，置于8～15℃低温中冷藏仍能存活，可供随时繁殖寄蝇的需要；家蚕饲养简便，花费少，而繁殖量又大，是一种较理想的替代寄主。

摘要: By using the domestic silkworm as the bioreactor (reorganizing the nucleus polygon virus expression system) to produce genetic products, we can attain our goal with less cost, higher productivity and activity in a safe manner. With the swelling development of biosciences and biotechnologies in recent years, we should develop new experimental species, especially the lower species, to replace the mammals and explore the animals' potential as a resource to benefit the human beings. We will make a detailed account of what we have achieved by utilizing the domestic silkworm exotic genes and the progress we have made in its animalization breeding technologies. Our domestic silkworm expression system is composed of its cells, transferring carrier, modified BmNPV, its chrysalis. We have obtained the rsj14NPV, which contains Japanese schistosome fatty acid albumen genes, and rsj28NPV, which contains Japanese schistosome 8KDGST genes. After being infected by the rsj14NPV, every silkworm (871×872) can produce 3.5 mgs' purified albumen. After the silkworm 57A.57B×24.26 is infected with Rsj28NPV, every silkworm will turn out 5mgs' purified albumen. By employing rSj14NPV(871×872),we observe the relations between the volume of contracted virus, the timing of the contraction, the duration of the virus and the amount of the produced albumen. The results are as follows: the silkworm that is inoculated with 4μL and 6μL produces 4-5mgs/silkworm, which is the highest, while the silkworm that is inoculated with 1μL group and 2μL group turns out only 1 mg, which is the lowest; the timing of the inoculation has no significant influence on the productivity; the reorganized virus that is preserved for a long period of time(9 months)is not very productive after being inoculated, which shows that we should use the required resurrected reorganized virus to achieve high productivity; by inoculating 55μLs’ resurrected reorganized virus(rsj14NPV) into the silkworm 871×872 will produce 4-5 mgs' albumen, which is similar to that of autumn wind ×white jade. In accordance with the current know how of sericiculture, we establish the common level silkworm breeding technical system, and set down the corresponding technical rules and disease tackling principles. The common level silkworm breeding technical system is composed of 6 technical links, that is, hatching hastening of the eggs; the picking and storing of mulberry leaves; breeding criteria of the 1 3 instar young silkworms; breeding criteria of the 4 5 instar silkworms; driving the silkworm onto a pile of straws to spin; antisepsis and preventing illness. We have confected the man made feeding stuffs for silkworms of all instars and the research on the feeding of the silkworm barrier system silkworm is still in progress. In the research, we will establish a technical system that can control the breeding environments and avoid pathogenic pollutions and the use of antibiotics. It can also carry out recycling breeding so as to meet the needs of silkworms of all kinds.

摘要: 【目的】鉴定家蚕Bombyx mori马氏管特异表达基因及其启动子，为家蚕的代谢排泄、解毒及免疫相关基因功能的研究及利用提供新的工具与支撑。【方法】基于家蚕全组织蛋白质组学数据进行马氏管特异表达基因筛选并利用RT-PCR验证基因在家蚕5龄幼虫头、表皮、中肠、脂肪体、马氏管、丝腺、精巢、卵巢、血细胞及气管中的表达；利用qRT-PCR检测家蚕不同发育阶段(卵、 3龄眠、 4龄幼虫、 5龄幼虫、上簇期、蛹和成虫)马氏管中及5龄幼虫头、表皮、中肠、脂肪体、马氏管、丝腺、精巢、卵巢、血细胞和气管中上述特异表达基因的表达量；利用PCR克隆家蚕马氏管特异表达基因BmPiT4175的上游启动子序列，并构建以EGFP为报告基因的转基因载体，获得转基因家蚕，在个体水平验证BmPiT4175上游启动子的活性及组织特异性。【结果】筛选获得了在家蚕马氏管特异表达的基因BmPiT4175;所克隆的BmPiT4175启动子长2 955 bp,其驱动的EGFP仅在转基因家蚕马氏管中特异表达，且EGFP与内源基因BmPiT4175的表达量相当。【结论】BmPiT4175的表达模式及其启动子活性都是家蚕马氏管特异的。