摘要: The pyrogen test is an important index of quality management relating to druggery injection and instillation and the current common methods are domestic rabbit test and Bacterial Endotoxins Test. Since there are shortcomings and limitations of resources in these two methods , it's necessary to look for another new substitute. We have done the preliminary study of the feasibility of the pyrogen test with domestic rabbit's blood plasm, human's plasm and THP 1. The principle is as follows: after incubating the standard sample of bacterial endotoxins, glycogen anti coagulation whole blood extracting from the healthy domestic rabbit, extralin anti congealable whole blood from healthy volunteers, THP 1 cells together, measure the releasing amount of TNF and IL 6 from cell gene by the method of ELISA as a endogenesis index judging the pro heat role of endotoxin. The results indicate: the blood rabbit's whole blood, human's whole blood, or THP 1cell, their releasing amount of TNF and IL 6 are closely connected with the amount of acting endotoxins within a certain range. Three methods have their own peculiarities and some feasibilities as a new substitute method for pyrogen test with domestic rabbit.

摘要: 目的　分析热原检查用家兔正常体温的动态变化。方法　按照有关规程对热原检查用家兔进行体温测定 ,并采用生物统计方法进行统计分析。结果　热原检查用家兔一年中正常体温 5— 7月份与 2— 4月份存在显著性差异 (P <0 0 5 ) ;二次用兔的正常体温平均值明显高于新兔的正常体温平均值 ,二者温差为 0 0 7℃ ,且存在显著性差异 (P <0 0 5 )。结论　热原检查用家兔正常体温随时间和使用状态而有所变化

摘要: 目的观察复方丹芩颗粒对动物眼玻璃体积血的影响。方法用家兔自体血造成眼玻璃体出血模型,分为复方丹芩颗粒(8 g/kg、4 g/kg、2 g/kg)治疗组和普罗碘胺注射液治疗组。用复方丹芩颗粒治疗8w,观察眼底可见度;兔眼病理切片中的巨噬细胞计数变化;玻璃体液中红细胞、血凝块、机化物检出率等情况。结果与模型组比较,复方丹芩颗粒组大鼠眼底可见度明显增加,眼底红细胞消失和溶解情况明显减轻,玻璃体液中红细胞、血凝块、机化物检出率明显减少,病理切片中的巨噬细胞计数明显升高。结论复方丹芩颗粒对瘀血有明显的解聚、促溶作用,增进红细胞溶解和吸收。促使巨噬细胞趋向眼部组织,增进积血的清除。

摘要: 目的探讨异维A酸胶丸对兔耳微痤疮的治疗作用。方法采用油酸给家兔耳外涂抹及表皮葡萄球菌皮下注射形成痤疮模型,再用异维A酸胶丸进行治疗。结果异维A酸胶丸有明显减小皮脂腺直径、毛囊面积作用。结论异维A酸胶丸对兔耳微痤疮具有治疗作用。

摘要: 目的改进胆囊炎动物模型的制备方法。方法在普通12.5 mm弯式止血钳钳齿的前1/3处,用工具在钳齿上锉出直径1～1.5 mm的孔道。结果用改制的止血钳钳夹胆囊壁及针头,在将注射器刺入胆囊抽出胆汁后,止血钳的孔道正好可以容纳针头和胆囊壁,而且针头可以较容易的抽出,而胆囊壁也不易脱出止血钳。这样就避免了菌液从胆囊中流入腹腔。结论用改进的方法制备家兔胆囊炎模型可降低腹腔内的感染,胆囊炎模型制备成功率较高。