摘要: 将SPF级Wistar大鼠从 3周龄断奶后饲养至 10周龄 ,分别测定 3周龄至 10周龄各周龄大鼠的血常规值和主要脏器重量系数及体重、头长、体长、尾长。结果表明 :随着周龄的增长 ,该大鼠血液RBC、HGB及HCT值逐渐升高 ;除肝脏外 ,各主要脏器重量系数逐渐降低 ;体重、头长、体长、尾长逐渐增长

摘要: 不同剂量激素对Wistar雌鼠超数排卵 ,并与正常雄鼠交配 ,收集 2 -细胞胚胎进行输卵管移植 ,收集 8-细胞胚胎进行子宫移植 ,结果发现每只Wistar雌鼠间隔 4 8h分别腹腔注射 5 0IU的PMSG与hCG ,Wistar大鼠的超数排卵效果最好 ,平均可获得 2 -细胞胚胎 36 .3± 15 8枚 ,输卵管移植后产仔率为 73 33% ,平均可获得 8-细胞 2 2 6±13 1枚 ,子宫移植产仔率为 75 0 0 %。

摘要: Monitoring method of biochemical markers is inferred corresponding gene changed according to protein changed in laboratory animals, and have definite limitation in some extent. But DNA fingerprints and microsatellite DNA directly monitored DNA of genetic materials, more accurate、more reliable and direct than biochemical markers. In the experiments six domestic strains(SHR、SHR/sp、WKY、LEW、RCS、F344) of inbred rat were analyzed DNA polymorphism using DNA fingerprints and microsatellite DNA, and set up the exact、reliable、speedy、convenient method of in the heredity monitoring of inbred rats. The result indicated:(1)DNA fingerprints: ①There were 14-19 distinguishable bands on 4.0kb-23.1kb every DNA fingerprinting. The similarity coefficient(F) and the probability of band sharing(X) were more than 0.9 in different individual of the identical inbred rat strain. and indicated more high similarity in genetic materials of the various individual in same group rats. ②The similarity coefficient and the probability of band sharing of DNA fingerprinting were less than 0.07, probability of the identical DNA fingerprinting was less than 10 -22 among the different individuals of different inbred strain. and indicated that there was more different in genetic materials of various individual in different inbred rat groups. ③)DNA fingerprinting obtained by four times experiment from same individual genome are almost coincident DNA fingerprinting from various tissues are also coincident in the same individual of 6 inbred rat strains. ④DNA fingerprinting of filial generation which RCS and LEW hybridized is analyzed, whose bands are found in their parents bands and complied with Mendel heredity law. (2) Microsatellite DNA:①DNA polymorphism was studied using microsatellite DNA method in 6 inbred rat strains. There is much polymorphism among the various rat strains;It was coincidence among the same inbred strains rat groups. ②10 microsatellite loci are analyzed in 6 inbred rat strains group. and the similarity coefficient is more higher (0.7) between SHR with SHR/sp、SHR and F344, which is more lower(0.1) between WKY with LEW and F344 and between LEW with RCS. DNA fingerprints was more polymorphism genetic marker in genome, there were a lot of merit in more bands analysed,monitoring more loci at the same time,more exact reliable,higher specificity and stability in the heredity of animal individuals, and was more better genetic marker. The length of amplification microsatellite DNA was shorter, microsatellite DNA was more sensitive,speedy,convenient than DNA fingerprints in microsatellite loci, then it was more applicable in monitoring of degraded material. In conclusion, DNA fingerprints and microsatellite DNA were more exact、reliable than biochemical markers, but they had a lot of merit shortcoming of every method, therefore selecting a suit method to monitor genetic material DNA of laboratory animals according to quantity,degree of accuracy,equipment and expense of monitoring and so on, and gratified monitoring demand.

摘要: 目的　探讨心气虚证动物模型的建立方法。方法　SD大鼠 4 0只采用基础进食量 2 4d ,每日按自身体重的5 %强迫负重游泳至力竭 ,大剂量灌服心得安 2 4mg 10 0g等综合方法建立心气虚证动物模型 ,其中 2 0只作为人参药物反证组。并设立正常对照组 4 0只 ,以进一步证明所建立模型是心气虚证动物模型。结果　与正常对照组比较 ,模型组大鼠心脏收缩功能和舒张功能均有所下降 ,反映虚证的SOD含量减少 ,MDA含量增加。而所有定量指标均发生了符合心气虚证的变化。结论　采用基础进食量、强迫负重游泳及大剂量灌服心得安的综合方法建立心气虚证动物模型是可行的

摘要: 目的　探讨戊巴比妥钠对大鼠诱导麻醉的适用剂量。方法　用无菌生理盐水配成 0 3%戊巴比妥钠溶液 ,按1mL 10 0g剂量进行腹腔麻醉。结果与结论　麻醉过程平稳 ,呼吸、心率正常 ,持续时间较长 ,苏醒快 ,计算方便 ,容易控制