摘要: Objective\ Pre transplant status of AS 30D hepatoma cell line was evaluated in Sprague Dawley rats. Cells from different sources, a) in vivo, from the other transplanted Sprague Dawley rats, b) in vitro, from cultured cell, and c) cryopreservation, from the liquid nitrogen storage tank, were transplanted into 5 week old female Sprague Dawley rats (respectively n=5, 3, and 5). Each animal received intraperitoneal injection of 0 5 ml cell suspension containing 1 5×10 6 cells. The results showed no significant difference among groups, in the quality parameters of cell transplantation, such as fluid volume, cell concentration, total cell, cell viability, and the daily consumption of food and water. However, the cell growth duration following the transplantation was lengthened at least one half fold or 5 days, when other sources of cells than the in vivo, were transplanted into rat abdominal cavity. Thus, the variety of pre transplant status of the AS 30D cells would enhance flexibility in cell preparations for the successful transplantation in different time frame.