摘要: Scleroderma is a connective tissue disease characterized by the fibrosisization of skin and internal organs.In this study,the genomic sequence and cDNA of Sjogren's syndrome/scleroderma autoantigen 1(SSSCA1)gene of the giant panda was cloned successfully using RT-PCR.The cDNA fragment cloned was 642 bp in length,and contained an open reading frame (ORF) of 600 bp encoding 199 amino acids.The genomic sequence was 1 262 bp,containing four exons and three introns. The deduced amino acid sequence showed that this protein was composed of 199 amino acids and its estimated molecular weight was 21.53841 kDa with a pI of 5.14. Three different patterns of functional sites were found:one protein kinase C phosphorylation site,five casein kinase Ⅱ phosphorylation sites,and three N-myristoylation sites. The SSSCA1 gene of the giant panda were highly homologous to those of some other mammals.
摘要: Based on bitter taste receptor T2R2 gene sequence of domesticated dog(AB249685), one pair of primers were designed and used to amplify an approximately 1.1 kb DNA fragment from genomic DNA sample of giant panda by using PCR. The PCR products were ligated into the pMD-18T vector, and then transformed into competent cells of E.coli DH5α. The identified positive clone was sequenced. The result showed that the T2R2 gene of giant panda was 1 008 bp in length, and contained complete exon, and 915 bp, encoding 304 amino acid residues. The pI of the protein is 9.56 and its molecular weight is 34.90 kDa. The prediction of topological structure for the protein indicated that it contained 11 potential functional sites(three N-glycosylation sites, three potential protein kinase C phosphorylation sites, two N-myristoylation sites, one Casein kinase II phosphorylation site, one cAMP- and cGMP-dependent protein kinase phosphorylation site and one Nuclear Localization Signal site) with five sites observed only in giant panda, and the protein comprised seven transmembrane helix regions, and four extracellular regions and four intracellular regions. The T2R2 was a hydrophobic protein with less hydrophilic components which mostly were located on the intracellular regions. Alignment analysis revealed that the homology of T2R2 gene nucleotide sequence of giant panda with that of dog, cat, cattle, horse, chimpanzee and mouse is 92.65%, 91.12%, 85.64%, 86.73%, 85.20%, 72.59%, respectively, and the homologies of amino acid sequence is 86.73%, 85.20%, 74.67%, 78.62%, 75.66%, 60.53%, respectively. On the whole, the giant panda T2R2 gene was high evolutionarily conserved, but its protein presented more abundant functional sites than did those of other species. However, the correlation between the characteristics of T2R2 gene and giant panda’s special diet needs to be further studied. The obtained sequences were submitted to GenBank, with accession NO.FJ812726.
摘要: Different dental morphological features,including the occlusal surface,interproximal surface,buccal surface and lingual surface,were described by observing the 155 non-isolated teeth in maxillary specimens from giant pandas(Ailuropoda melanoleuca) from the Qinling Mountains.The correlation between dental features and diet was discussed based on the measurement of dental crown width,dental crown thickness,dental crown height,dental cervical width,dental cervical thickness,dental root length and dental length,in parallel with a study on the feeding ecology of giant pandas.Dental unity of morphology and function of wild giant pandas was also described.The correlation between dental anatomy and physiology was further analyzed.The results showed that during the evolutionary process,as a special stock of carnivore,the giant panda's food was highly specialized.Its dental morphological features are significantly different from those of other carnivores and have their own peculiarities.The incisor has a trend of degradation and double dentition.It has lost the dental morphological features of other carnivores.The canine is strong and forms a cone.Its crown height is twice the height than the molar's crown.The premolar and the molar have complex dental morphology.Their occlusal surface has many crista.The cingulum is well-developed.The broad and uneven occlusal surfaces are similar to those of omnivorous and herbivorous animals.Changes in the food structure affect the dental masticatory function in different ways,resulting in the adaptive performance in the dental morphology.Morphological characteristics of these teeth meet the needs of masticatory function,which is closely related to its oral physiological functions.The results of this study provide the anatomical basis for the prevention and treatment of dental disease of giant pandas,as well as having very important ecological significance.
摘要: The multisubunit NADH ubiquinone oxidoreductase (complex I) is the first enzyme complex in the electron transport chain of mitochondria. The iron-sulfur protein (IP) fraction is made up of 7 subunits, including NDUFS6 (NADH-ubiquinone oxidoreductase Fe-S protein 6). In order to understand the giant panda’s NDUFS6 gene and coding of protein using RT-PCR combined with in silico cloning, we isolated and sequenced the cDNA encoding NADH-ubiquinone oxidoreductase iron-sulfur protein 6 of the giant panda (Ailuropoda melanoleuca).The deduced protein sequence showed that the protein is composed of 124 amino acids and the estimated molecular weight of the NDUFS6 protein is 13.632 kDa with an isoelectric point (PI) of 9.51. Alignment analysis reveals that the deduced protein sequence shares 85.6 %,82.4 %,76.6 % and 76.1 % homology with that of Homo sapiens, Bos taurus, Rattus norvegicus and Mus musculus, respectively. In particular, there are 2 Protein kinase C phosphorylation sites (14SGK16 and51SRK53) in the NDUFS6 protein of the giant panda.